Microbiological Factors and Inflammatory Cytokines in Acute and Chronic Rhinosinusitis

Objective: We aimed to use middle meatus aspiration technique (MMAT) in both microbiological diagnosis and detection of cytokines and to compare the groups having ARS and non-polyp CRS after determining the microbiological agents and  the levels of IL-4, IL-5, IL-13, IL-32, TGF-beta, and thymic stromal lymphopoietin (TSLP).

Material and Methods: The patients were classified as ARS and non-polyp CRS. The microbiological samples were grown on 5% defibrinated sheep blood agar, chocolate agar, Eosin-Methylene Blue agar (EMB), and Sabouraud- dextrose agar (SDA) media. IL-4, IL-5, IL-13 IL-32, TLR2, TSLP, and TGF-beta levels of biochemical samples were studied by ELISA method using commercial kits. 

Results: Samples of nasal discharge were collected in a total of 44 patients. The difference between ARS Group and Non-polyp CRS Group regarding the culture growth of pathogens was statistically significant. The levels of TSLP, TGF-beta, IL-4, IL-5, IL-13 and IL-32 were not in conformity with a normal distribution. The differences between ARS Group and Non-polyp CRS Group were not significant regarding these variable. 

Conclusion: We have the opinion that, MMAT is a non-invasive method that can be performed at the circumstances of an outpatient clinic and can be used for microbiological diagnosis as well as routine identification of inflammatory processes

Cite this article as: Başak S, Telli M, Başal Y, Yenisey Ç. Microbiological Factors and Inflammatory Cytokines in Acute and Chronic Rhinosinusitis. Eur J Rhinol Allergy 2021; 4(1): 16-20.